Quattuordecim genera pathogenorum respiratoriorum coniuncta
Nomen producti
HWTS-RT159B Quattuordecim Genera Pathogenorum Respiratoriorum Instrumentum Detectionis Acidi Nucleici Coniunctum (PCR Fluorescens)
Epidemiologia
Infectio tractus respiratorii est morbus in hominibus frequentissimus, qui in quolibet genere, aetate et regione occurrere potest. Una ex causis praecipuis morbiditatis et mortalitatis in populatione toto orbe terrarum est.[1]Pathogena respiratoria communia includunt novum coronavirus, virus influenzae A, virus influenzae B, virus syncytiale respiratorium, adenovirus, metapneumovirus humanum, rhinovirus, virus parainfluenzae typi I/II/III/IV, bocavirus, enterovirus, coronavirus, mycoplasma pneumoniae, chlamydia pneumoniae, et streptococcus pneumoniae, et cetera.[2,3].
Canalis
| Positio Putei | Nomen Solutionis Reactionis | Pathogena Detegenda |
| 1 | Magister Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Magister Mix B | Adv, hMPV, MP, Cpn |
| 3 | Magister Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Magister Mix D | CoV, EV, SP, imperium internum |
| 5 | Magister Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Magister Mix B | Adv, hMPV, MP, Cpn |
| 7 | Magister Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Magister Mix D | CoV, EV, SP, imperium internum |
Parametri Technici
| Repositorium | ≤-18℃ |
| Tempus conservationis | Novem menses |
| Typus Speciminis | Peniculus oropharyngeus, peniculus nasopharyngeus |
| Ct | ≤38 |
| CV | <5.0% |
| Limitatio Detectionis (vel Limitatio Detectionis) | 200 Exemplaria/mL |
| Specificitas | Resultata probationis reactionis transversalis demonstraverunt nullam reactionem transversalem inter hoc instrumentum et Cytomegalovirus, virus Herpes simplex typus 1, virus Varicella-zoster, virus Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillum, Legionella pneumophila, Moraxella catarrhalis, stirpes attenuatas Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida exstitisse. *Antibacterium albicans*, *Rothia mucilaginosa*, *Streptococcus oralis*, *Klebsiella pneumoniae*, *Chlamydia psittaci*, *Coxiella burnetii* et acida nucleica genomica humana. |
| Instrumenta Applicabilia | Systema PCR Temporis Realis SLAN-96P (Societas Technologiae Medicae Hongshi, Ltd.)Systema PCR Temporis Realis 7500 Biosystems Applied Systema PCR Temporis Realis Celeris Applied Biosystems 7500 QuantStudio®Quinque Systemata PCR Temporis Realis Systema Detectionis PCR in Tempore Reali LineGene 9600 Plus (FQD-96A, technologia Hangzhou Bioer) MA-6000 Therma Cyclator Quantitativus Realis Temporis (Suzhou Molarray Co., Ltd.) Systema PCR Temporis Realis BioRad CFX96, Systema PCR Temporis Realis BioRad CFX Opus 96 |
Fluxus Operis
Reagentium extractionis commendatum: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (quod cum Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010) adhiberi potest) a Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Volumen exempli extracti est 200µL. Gradus subsequentes secundum instructiones ad usum huius reagentis extractionis peragendi sunt. Volumen elutionis commendatum est...80µL.
