Quattuor Genera Virusorum Respiratoriorum
Nomen producti
HWTS-RT099 - Instrumentum ad Quattuor Genera Virusorum Respiratoriorum Acidi Nucleici Detegendos (PCR Fluorescens) - Systema PCR Temporis Realis NED-ABI 7500/ABI 7500 Systema PCR Celeria Temporis Realis/QuantStudio®Quinque Systemata PCR Temporis Realis
HWTS-RT158-4 Genera Virusorum Respiratoriorum Instrumentum Detectionis Acidi Nucleici (PCR Fluorescens) -Quasar 705
Epidemiologia
Morbus Coronaviralis anni 2019, "COVID-19" appellatus, pneumoniam a...2019-nCoVinfectio.2019-nCoVest coronavirus generis β pertinens. COVID-19 est morbus infectiosus respiratorius acutus, et populatio plerumque susceptibilis est. In praesenti, fons infectionis sunt praecipue aegroti infecti ab...2019-nCoV...et personae infectae asymptomaticae fons infectionis fieri possunt. Secundum investigationem epidemiologicam hodiernam, tempus incubationis est 1-14 dies, plerumque 3-7 dies. Febris, tussis sicca et lassitudo sunt manifestationes principales. Pauci aegroti symptomata habebant.qualia suntcongestio nasalis, rhinorrhea, dolor faucium, myalgia et diarrhoea, cetera.
Canalis
| FAM | MMXIX-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Imperium Internum |
Parametri Technici
| Repositorium | -18℃ |
| Tempus conservationis | Novem menses |
| Typus Speciminis | Peniculus oropharyngeus |
| Ct | ≤38 |
| Limitatio Detectionis (vel Limitatio Detectionis) | 2019-nCoV: 300 Exemplaria/mLVirus influenzae A/Virus influenzae B/Virus syncytialis respiratorius: 500 exemplaria/mL |
| Specificitas | a) Resultata reactionis transversalis ostendunt nullam reactionem transversalem inter apparatum et coronavirus humanum SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, virus parainfluenzae typi 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus humanum, enterovirus A, B, C, D, virus pulmonarium humanum, virus Epstein-Barr, virus morbillorum, virus cytomegalovirus humanum, rotavirus, norovirus, virus parotitidis, virus varicellae-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, aspergillum fumarium, candidam albicans, candidam glabratam, pneumocystis jiroveci et neonatum. Cryptococcus et acidum nucleicum genomicum humanum. b) Facultas anti-interferentiae: mucinum selectum (60mg/mL), 10% (v/v) sanguinis et phenylephrinum (2mg/mL), oxymetazolinum (2mg/mL), natrii chloridum (praeservativis inclusis) (20 mg/mL), beclomethasonum (20mg/mL), dexamethasonum (20mg/mL), flunisolidum (20μg/mL), triamcinoloni acetonidum (2mg/mL), budesonidum (2mg/mL), mometasonum (2mg/mL), fluticasonum (2mg/mL), histamini hydrochloridum (5mg/mL), interferon alpha (800IU/mL), zanamivirum (20mg/mL), ribavirinum (10mg/mL), oseltamivirum (60ng/mL), peramivirum (1mg/mL), lopinavirum (500mg/mL). ritonavirum (60mg/mL), mupirocinum (20mg/mL), azithromycinum (1mg/mL), ceftriaxonum (40μg/mL), meropenemum (200mg/mL), levofloxacinum (10μg/mL) et tobramycinum (0.6mg/mL) ad experimentum interferentiae adhibita sunt, et eventus ostendunt substantias interferentes cum concentrationibus supra dictis nullam reactionem interferentiae ad eventus experimentorum pathogenorum habere. |
| Instrumenta Applicabilia | Systema PCR Temporis Realis Applied Biosystems 7500Systema PCR Temporis Realis Celeris Applied Biosystems 7500 Systema PCR Temporis Realis QuantStudio®5 |
Fluxus Operis
Optio 1.
Instrumentum Macro & Micro-Test Viral DNA/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) et Extractor Automaticus Acidi Nucleici Macro & Micro-Test (HWTS-3006) a Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. productum. Volumen exempli extracti est 200μL, et volumen elutionis commendatum est 80μL.
Optio II.
QIAamp Viral RNA Mini Kit (52904) a QIAGEN productum, vel Nucleic Acid Extraction or Purification Kit (YDP315-R) a Tiangen Biotech (Beijing) Co., Ltd. productum. Volumen exempli extracti est 140μL, et volumen elutionis commendatum est 60μL.
