Carbapenem Resistentia Gene (KPC / NDM / Oxa XLVIII / XXIII OXA / Vim / AB)
Product Name
HWTS-ot045 carbapenem resistentia gene (KPC / NDM / XLVIII OXA / XXIII OXA / Vim / AB) PRC) PCR)
Epidemiology
Carbapenem antibiotics sunt atypical β-Lactam antibiotics cum latior antibacterial spectro et fortissimum antibacterial operatio. Propter eius stabilitatem ad β-lactamase et humilis toxicity, id est unum ex maxime momenti antibacterial medicinae ad curatio gravi bacterial infectiones. Carbapenems sunt valde firmum ad plasmid-mediante extenditur, spectro β-lacamases (ESBLS), chromosomes, et plasmid-mediated Cephalosporinases (Ampc enzymes).
Channel
| PCR-MIX I | PCR-Nobis II | |
| Fam | Im | Vim |
| Vic / Hex | Internum | Internum |
| CYV | Ndm | KPC |
| ROX | Oxa48
| Oxa23 |
Technical parametri
| Repono | ≤, XVIII ℃ |
| PLUTEUM-vitae | XII mensibus |
| Specimen Type | Sputum, pura coloniarum, rectalis swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LOD | 103CFU / ml |
| Specifica | a) in ornamentum detects in standardized turba negative references, et eventus occursum de requisitis correspondentes references. b) Eventus Crucis-Reactivity test ostenderet quod hoc ornamentum habet crucem reactionem cum aliis respiratoriorum patriar, ut Klebsiella pneumoniae, Acinetobacter, ut Klebsiella Pseuditide, Streptococcus Pneumoniae, Pseuditia, Streptococcus, Pseuditia, Streptococcus, Pseuditia Meningitidis, Haemophilus, Klebsiella Oxytoca, Haemophilus, Klsiella Oxytoca, Haemophilus, Klsiella Oxytoca, Haemophilus, Haemophilus, Klebsiella Influenzae, Acinetobacter Junii, Acinetobacter Haemolyticus, Legionella Pneumophila, Escherichia Coli, Pseudomonas Fluoromens, Candida Albicans, Chlamydia Pneumoniae, respiratoriorum adenovirus, entococcus, aut exempla, SME, SHV, SME, SME, SHV, SME, SME, SHV, SME, SME, SHV, SME, SME, SHV, SME, SME, SHV, TEM, etc c) anti-intercessiones: Mucin, Minocycline, Gentamicin, Clindamycin, imipenem, cefoperazone, Meropenem, ciprofloxacin hydrochloride, levofacin, clavulanic acidum, et eventus non intercedendo intercessione substantiarum non intercedente reactionem ad deprehendatur Carbapenem resistentia genes KPC, NDM, OXA48, OXA23, Vim et Imp. |
| COMPRESSUS | Applicari Biosystems (VII) D Real-vicis PCR Systems QuantStudio®5 Real-vicis PCR Systems Slan-96P Real-vicis PCR systems (Hongshi Medical Technology Co., Ltd.) LightcyCler®®®® Real-vicis PCR ratio Linenene (IX) DC plus realis-vicis PCR deprehensio ratio (FQD, 96a,HangzhouBioer Technology) MA-(VI) Real-vicis quantitatis scelerisque cycler (Suzhou Molalarray Co., Ltd.) BIOD CFX96 Real-vicis PCR ratio BIOD CFX Opus XCVI Real-vicis PCR ratio |
ADMODO
Optio I.
Commendatur extraction Reagent: Macro & Micro-test Generalis DNA / RNA Kit (HWTS, CCCI9-50, HWTS, CCCI9-32, HWTS, CCCI9-48, HWTS, CCCI9-96), (quod potest esse cum Macro & micro-test Autedic Nucleiic Acidum extractor (HWTS-3006c, HWTS-3006b)) by Jiangsu Macro & Micro-Test Med-Tech Macro, .. Add 200μl Et Thallus praecipiti. Subsequent gradibus sequi instructiones extractionem et commendatur Lorem volumine100μl.
Optio II.
Commendatur extraction Reagent: nucleic acidum extraction vel purificationem Reagent (ydp302) by Tiangen (Beijing) Co., Ltd. de Extraction ut coepi in stricto ad gradus II ad Tallus praecipitari Et excutite usque ad Thallus omnino suspensus). Uti RNase / DNase Aquam ad Helium et Ecommended Helium volumen est 100μl.
Optio III.
Commendatur extraction Reagent: Macro & Micro-test sample dimittis Reagent. Sputum sample indiget lavari per addendo 1ml de normalis SAL ad superius dicta Tractatus Thallus praecipiti, centrifuged at 13000R / min pro V minutes, et supernatant est (servo 10-20μl supernatanti). Nam pura colonia et rectalis swab, adde 50μl of sample dimittere se directe ad superius dicta tractata Thallus praecipitari, et subsequent gradus extrahi secundum disciplinam ad usum.
