Carbapenem Resistentia Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Product nomen
HWTS-OT045 Carbapenem Resistentia Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Detection Kit (Fluorescens PCR)
Epidemiologia
Carbapenem antibiotici sunt atypici β-lactam antibiotici cum latissimo spectro antibacterial et validissima actione antibacterial.Propter suam stabilitatem ad β-lactamasem et toxicitatem humilis, una ex maximis medicamentis antibacterial factus est ad tractationem gravium contagionum bacterial.Carbapenema valde stabilia sunt ad spectrum plasmid-mediatum extensum β-lactamases (ESBLs), chromosomata, et cephalosporinases plasmid-mediatas (AmpC enzymes).
Channel
| PER-MIX 1 | PER-MIX 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Internum Imperium | Internum Imperium |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Technical Parameters
| Repono | ≤-18℃ |
| Fasciae vita | XII mensibus |
| Specimen Type | Sputum, coloniae purae, rectal swab . |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Specification | a) Ornamentum detegit normas notas societatis negativas, eventusque requisitis congruentium indiciorum. b) Eventus experimenti crucis-reactivitatis ostendunt hoc ornamentum crucis non habere reactionem cum aliis pathogenis respiratoriis, ut Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Acinetobacterae aeruginosa, Haemophilus influentia. junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, respirator adenovirus, Enterococcus, seu exemplaria alia medicamentorum repugnantium generum continens CTX, MECA, SME, SHV, TEM, etc. c) Anti-impedimentum: Mucin, Minocyclinum, Gentamicin, Clindamycin, Imipenem, Cefoperazonem, Meropenem, Ciprofloxacin Hydrochloridem, Levofloxacin, Acidum Clavulanicum, Roxithromycinum ad probationem impedimenti delecti sunt, et eventus ostendunt substantias praedictas impedimento nullum habere reactionem. ad detectionem carbapenem resistentiae genes KPC, NDM, OXA48, OXA23, VIM, IMP. |
| Applicabilis Instrumenta | Acta Biosystems (VII)D Real-time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-time PCR systema LineGene (IX)DC Plus Real-time PCR Detection System (FQD-96A.HangzhouBioer technologia) MA-6000 Real-time Quantitatis Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-time PCR System BioRad CFX Opus 96 Real-time PCR System |
Opus O
Optio 1 .
Commendatur extraction reagens: Macro & Micro-test Generalis DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (quod adhiberi potest cum Macro & Micro-test Automatico Nucleico Acido Extractor (HWTS-3006C, HWTS-3006B)) a Jiangsu Macro & Micro-test Med-Tech Co., Ltd. Add 200μL salinarum normalium ad Thallus praecipitanter.Sequentes gradus instructiones ad extrahendam sequi debent, et volumen commendabile elutionis est .100μL.
Optio II.
Extraction reagens commendatur: Acidum nuclei extractio seu Purificatio Reagenti (YDP302) a Tiangen Biotech (Beijing) Co., Ltd. Extractio stricte incipienda est secundum gradum 2 instructionis ad usum (200μL quiddam GA ad thallum praecipitatum add. et excutite donec thallus penitus suspendatur).Utere RNase/DNase aqua libera pro elutione, et volumen commendabile elutionis 100μL est.
Optio III.
Commendatur extraction reagens: Macro & Micro-Test Sample Release Reagent.Sputum specimen lavari debet addendo 1mL salinarum normalium ad praedictam thallum tractatam praecipitatam, centrifugium ad 13000r/min per 5 minuta, et supernatans abicitur (custodia 10-20µL supernatantis).Ad puram coloniam et swab rectalem, 50µL specimen remissionis reagenti rectae ad praedictos tractatos thallum praecipites adde, et sequentes gradus extrahi debent secundum instructionem ad usum.
