Mycobacterium tuberculosis nucleic acidum et rifampicin resistentia
Product Name
HWTS, RT074B, Mycobacterium tuberculosis nucleic acidum et rifampicin resistentia deprehendatur ornamentum (liquefactio curva)
Certificatorium
CE
Epidemiology
Mycobacterium tuberculosis, paulo tuberculum Bacillus, TB est in pathogenic Bacteria, quae causas tuberculosis. Currently, in communiter primo-linea anti-tuberculosis medicamentis includit Isoniazid, rifampicin et hexambutol, etc secunda-linea anti-tuberculosis medicamentis includit fluorequinolones, Amikacin et Kanamycin, etc. novus developed medicamina et Canamycin, etc. et delamini, et etc. . Tamen, propter falsa usum anti-tuberculosis Medicamenta et characteres in cellula murum structuram de Mycobacterium tuberculosis, Mycobacterium tuberculosis develops medicamento resistentia ad anti-tuberculosis medicamentis, quod brings gravis challenges ad ne et curatio tuberculosis.
Rifampicin est late usus est in curatio pulmonalis tuberculosis aegris cum nuper 1970s et habet significant effectus. Hoc fuit primum arbitrium ad breviare chemotherapy pulmonalis tuberculosis aegris. Rifampicin resistentia maxime per mutationem RPOB gene. Licet nova anti-tuberculosis medicaménta sunt constanter egredientur, et orci efficaciam pulmonis tuberculosis aegris etiam continued ad amplio, ibi adhuc relativus anti-tuberculosis medicamentis, et relative in irrationali medicamento usus est in orci et relative altum. Plane, in Mycobacterium tuberculosis in aegris cum pulmonalis tuberculosis non potest omnino occidit opportune modo, quod eventually ad diversas gradus medicamentis resistentia in patientis corporis, prolonges cursu morbi auget periculo mortis patientis.
Channel
| Channel | Channels et fluorophores | Reactionem quiddam est | Reactionem buffer b | Reactionem quiddam c |
| Fam Channel | Notario: Fam, Quencher: Nihil | RPOB 507-514 | RPOB 513-520 | 38kd et is6110 |
| CYV | Notario: Cy5, Quencher: Nihil | RPOB 520-527 | RPOB 527-533 | / |
| Hex (Vic) Channel | Notario: Hex (Vic) Quencher: Nihil | Internum | Internum | Internum |
Technical parametri
| Repono | ≤, XVIII ℃ in tenebris |
| PLUTEUM-vitae | XII mensibus |
| Specimen Type | Sputum |
| CV | ≤5.0% |
| LOD | Mycobacterium tuberculosis L Bacteria / ml Rifampicin-repugnans ferox Type: 2x103Bacteria / ml Homozygous Mutant: 2x103Bacteria / ml |
| Specifica | Hoc detegit fera-type Mycobacterium tuberculosis et mutationem sites alia medicamento resistentia genes ut katg 315g> c \ a, ad 15c> T, in test eventus non resistentia ad RIFAMPIn, quod significat non est crucem, reactivity. |
| Applicabiles instrumenta: | Slan-96P realis-vicis PCR systems BIOD CFX96 Real-vicis PCR ratio LightcyCler480® Real-vicis PCR ratio |
ADMODO
Si uti Macro & Micro-test Generalis DNA / RNA Kit (HWTS, 3019-50, HWTS, 3019-32, HWTS, 3019-48, HWTS-3019-96) (qui possunt esse cum Macro & Micro-test automatic Nucleic Acidum extractor (HWTS, 3006C, HWTS, 3006b)) aut Macro & Micro-test viral DNA / RNA Columna (HWTS, 3022-50) by Jiangsu Macro & Micro-test med-tech Co., Ltd ad extractionem, addere 200μl de positivo potestate, negative et processionaliter sputum sample est probata in sequence et adde 10μl Internum imperium seorsum in positivum imperium, negans imperium et processionaliter sputum sample ut probata, et subsequens gradibus debet esse proprie ferri ex extraction instructiones. Extractum sample volumine est 200μl et commendatur amet volumine est 100μl.
