Clostridium difficile toxin A/B gene（C.diff）
Product nomen
HWTS-OT031A Nuclei Acidum Detection Ornamentum Clostridium difficile toxin A/B gene (C.diff) (Fluorescens PCR)
Testimonium
CE
Epidemiologia
Clostridium difficile (CD), gram-positivum anaerobicum sporogenicum Clostridium difficile, est unum e praecipuis pathogenis causantibus infectiones nosocomiales intestinales.Amet, circiter 15%~25% diarrhoeae antimicrobialis relatae, 50%~75% colitis antimicrobialis actis et 95%~100% enteritis pseudomembranacei causatur per infectio difficilem Clostridium (CDI).Clostridium difficile est pathogen conditionale, inter modos toxigenicos et modos non toxigenicos.
Channel
| FAM | tcdAgene |
| ROX | tcdBgene |
| VIC/HEX | Internum Imperium |
Technical Parameters
| Repono | ≤-18℃ |
| Fasciae vita | XII mensibus |
| Specimen Type | scabellum |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Specification | hoc ornamento utuntur ad deprehendendas alios pathogenis intestinales sicut Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenicum modos, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus et genomic humanum. DNA, eventus omnes negativi sunt. |
| Applicabilis Instrumenta | Acta Biosystems (VII)D Real-time PCR Systems Acta Biosystems (VII)D Fast Verus-Tempus PCR Systems QuantStudio®5 Real-time PCR Systems SLAN-96P Real-time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-time PCR system LineGene (IX)DC Plus Verus-Tempus PCR Detection System(FQD-96A.HangzhouBioer technologia) MA-6000 Real-time Quantitatis Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-time PCR System BioRad CFX Opus 96 Real-time PCR System |
Opus O
Optio 1 .
Adde 180μL lysozyme quiddam ad praecipitatum (lysozymum ad 20mg/mL diluendum cum lysozymo diluente), pipettam bene miscere, et processum ad 37°C plusquam 30 minuta. Accipe 1.5mL tubi centrifugii RNAse/DNase-liberi; et adde180μL imperium positivum et imperium negativum in ordine.Add10μL imperium internum ad specimen probandum, imperium positivum, et imperium negativum in ordine, ac utere Acidum nuclei extractionis seu Purificationis Reagenti (YDP302) a Tiangen Biotech (Beijing) Co., Ltd. pro subsequenti sample DNA extractione, et Quaeso stricte sequi instructiones ad usus proprios gradus.Usus DNase / RNAse liberum H2O pro elution, et volumen commendabile est 100μL.
Optio II.
Accipe 1.5mL tubus centrifugii RNAse/DNase-liberi, et adde 200μL imperium positivum et imperium negativum in ordine.Add10μL imperium internum ad specimen probandum, imperium positivum, et imperium negativum in ordine, ac Macro & Micro-test Viralis DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) et Macronem & Micro-test Automaticum Acidum Nucleicum Extractum (HWTS-3006).Extractio stricte exercenda est secundum instructionem ad usum, et volumen commendatur elutionis 80μL.
