Clostridium difficile toxin a / b gene (c.diff)
Product Name
HWTS-ot031a nucleic acidum deprehendatur Kit ad Clostridium difficile toxin a / b gene (c.diff) (fluorescence PCR)
Certificatorium
CE
Epidemiology
Clostridium (CD), A Gram, positivum Anaerobic sporogenic Clostridium difficile, est unum de principalis pathogens causing nosocomial intestinorum infectiones. Clinically, de XV% ~ XXV% de Antimicrobial-Related Diarrhea, L% ~ LXXV% of Antimicrobial-Related Colitis et XCV% C% of Pseudomembranous ENTITIS et Clostridium C% of Pseudomembranous ENTITIS per Clostridium CRUSTA PREFECTIMEMBRANUS ETTITIS causatur a Clostridium infectio (CDI). Clostridium difficile est conditionalis pathogen, comprehendo toxigenic stranulis et non-toxigenic modos.
Channel
| Fam | tcdagene |
| ROX | tcdbgene |
| Vic / Hex | Internum |
Technical parametri
| Repono | ≤, XVIII ℃ |
| PLUTEUM-vitae | XII mensibus |
| Specimen Type | sella |
| Tt | ≤38 |
| CV | ≤5.0% |
| LOD | 200CFU / ml |
| Specifica | Utere hoc ornamentum ut deprehendere alias intestinorum pathogens ut Esaichia coli, Staphylococcus aureus, Shigella, salmonella, Vibrio parahaemolyticus, Group Brightococococococcus, Rotavirus non-Group B Streptocococcus, Rotavirus, Norovirus, et Humanum, Geom DNA, eventus Omnes enim negans. |
| COMPRESSUS | Applicari Biosystems (VII) D Real-vicis PCR Systems Applicari Biosystems (VII) D ieiunium realis-vicis PCR systems Quantstudio®V Real-vicis PCR Systems Slan-96P Real-vicis PCR systems (Hongshi Medical Technology Co., Ltd.) LightCycler®CDLXXX realis-vicis PCR ratio Linenene (IX) DC plus realis-vicis PCR deprehensio ratio (FQD, 96a,HangzhouBioer Technology) MA-(VI) Real-vicis quantitatis scelerisque cycler (Suzhou Molalarray Co., Ltd.) BIOD CFX96 Real-vicis PCR ratio BIOD CFX Opus XCVI Real-vicis PCR ratio |
ADMODO
Optio I.
Addere 180μl de Lysozyme quiddam ad praecipiti (dilute in Lysozyme ad 20mg / ml cum lysozyme diluent), pipette miscere bene et processum ad XXXVII ° C plus quam XXX minuta 1.5ml de rnase (DNase-liberum Centrifuge Tuba, et add180μl of positivum imperium et negans imperium in sequence. Addo10μl of internum potestate ad sample ut probata, positivum imperium, et negans imperium in serie, et uti nucleic acidum extraction vel purificationem Reagent (ydp302) per Tiangen Biote (Beijing), Ltd. et subsequent sample DNA extraction, Quaeso se sequi instructiones ad usum specifica gradibus. Usus DNase / RNase Free H2O enim eludendi et commendatur amet volumine est 100μl.
Optio II.
Accipere 1.5ml de RNase / DNase-liberum Centrifuge fistulam, et addere 200μl of positivum imperium et negans imperium in sequence. Addo10μl of internum control ad sample ut probata, positivum imperium, et negans imperium in sequence et uti Macro & Micro-test viral DNA / RNA, 3004-48, HWTS, 3004- XCVI) et Macro & Micro-test automatic nucleic acidum extractor (HWTS, MMMVI). Extractionem debet ferri in stricto normam disciplinam ad usum, et commendatur Electio 80μl.
