Zaire Ebola Virus
Product nomen
HWTS-FE008 Zaire Ebola Virus Nucleic Acidum Detection Kit (Fluorescens PCR)
Epidemiologia
Virus Ebola ad Filoviridas pertinet, quod est virus RNA negativo-stratum insegmentosum.Virus filamenta longa sunt cum mediocris virionis longitudine 1000nm et diametro circiter 100nm.Virus Ebola genome insegmen-stratum negativum est RNA cum magnitudine 18.9kb, descriptam 7 servo structuris et dapibus non-structuralibus.Virus Ebola in genera dividi potest sicut Zaire, Sudan, Bundibugyo, Tai Silva et Reston.Inter eos, typus Zaire et genus Sudan delatae sunt causa multorum hominum ex contagione mortibus.EHF (febris Ebola hemorrhagia) est morbus acutus hemorrhagicus contagiosus per virus Ebola causatus.Homines maxime contactu inficiuntur cum humoribus, secretionibus et excretis aegrorum vel animalium infectis, ac manifestationes clinicae maxime prominentes febres, sanguinem sanguinis et damnum multiplex organi.EHF mortalitatis rate of 50%-90% habet.
Channel
| FAM | MP Acidi nuclei |
| ROX | Internum Imperium |
Technical Parameters
| Repono | ≤-18℃ |
| Fasciae vita | IX mensibus |
| Specimen Type | Nova serum、Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copia/μL |
| Specification | Utere kits ad probationes societatis negativas, eventus metus. |
| Applicabilis Instrumenta | Acta Biosystems (VII)D Real-time PCR Systems Acta Biosystems (VII)D Fast Verus-Tempus PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-time PCR systema LineGene 9600 Plus Verus-Tempus PCR Detection System(FQD-96A, Hangzhou Bioer technology) MA-6000 Real-time Quantitatis Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, and BioRad CFX Opus 96 Real Time PCR System |
Opus O
Optio 1 .
Commendatur extraction reagens: QIAamp Viral RNA Mini Kit (52904), Acidum Nucleicum Extraction seu Purificationis Reagenti (YDP315-R) Tiangen Biotech (Beijing) Co.,Ltd.Extrahi debet stricte ad normam praeceptorum, et extractio exemplaris commendaticii est 140μL, et commendatur elutionis volumen 60μL.
Optio II.
Extraction reagens commendatur: Macro & Micro-test Viralis DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) et Macro & Micro-test Automatic Extractor Nucleici Acidi (HWTS-3006). extrahi secundum mandatum.Extractio voluminis specimen 200μL est, et volumen commendabile 80μL est.
Optio III.
Commendatur extractio reagentium: Extractio Acidum nuclei Reagenti (000020261) et Praecipuum Systematis Praeparationis Sample Automated (MGISP-960) ab BGI extrahendum secundum instructiones.Extractio voluminis 160μL est, et volumen commendabile est 60μL.
