14 Humana Papillomavirus genera summus periculo (16/18/52 Typing)
Product nomen
HWTS-CC019A-14 Genera summi periculi Humani Papillomavirus (16/18/52 Typing) Acidi Nucleici Detection Ornamentum (Fluorescens PCR)
Epidemiologia
Studiorum monstraverunt HPV contagiones persistentes et plures contagiones unum e praecipuis causis cancri ceruicis.In statu, curationes efficaces agnitae adhuc carent propter cancer ceruicis ab HPV causatis, ideo prima inventio et prohibitio contagionis ceruicis ab HPV causata est clavis ad cancrum ceruicis impediendum.Magni momenti est constituere diagnosticam diagnosticam experimentum simplex, specifica et celeri diagnosi et curatio cancer ceruicis.
Channel
| Channel | Type |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68; |
| CY5 | HPV 52 |
| Quasar 705/CY5.5 | Internum Imperium |
Technical Parameters
| Repono | ≤-18℃ |
| Fasciae vita | XII mensibus |
| Specimen Type | Urina ceruicis Swab，Vaginal Swab |
| Ct | ≤28 |
| LoD | 300 Copia/mL |
| Specification | Nulla crux-reactividad cum aliis speciminibus respiratoriis ut Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q febris, Chlamydia pneumoniae, Adenovirus, Virus syncytialis respiratorii, Parainfluenza 1, 2, 3, virus Coxsackie, virus Echo, Metapneumovirus A1/A2/ B1/B2, Virus syncytialis respiratorii A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Virus Boca 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. et genomicum humanum DNA. |
| Applicabilis Instrumenta | MA-6000 Real-time Quantitatis Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System and BioRad CFX Opus 96 Real-time PCR System |
Opus O
1.Urina sample
A: Accipe1.4mL de specimen urinae probandum et centrifugium in 12000rpm pro 5 minutis;supernatantem abiicias (commendatur ut 10-20μL supernatantem ab imo e tubo centrifugis), 200μL adde exemplum reagens emissio Reagent (HWTS-3005-8).
B: Accipe1.4mL urinae specimen probetur et centrifugium in 12000rpm pro 5 minutis;supernatantem abiicias (commendatur ut 10-20μL supernatantis ab imo tubi centrifugii custodiant), et 200μL salinarum normalium resuspendendi, sicut specimen probandum, adde.Subsequens extractio fieri potest cum Macrone & Micro-teste Virali DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (quod adhiberi potest cum Macro & Micro-test Automatic Acidum Nucleicum Extractor (HWTS-3006C, HWTS-3006B)) Jiangsu Macro & Micro-test Med-Tech Co., Ltd. stricte ad normam instructionis.s ad usum.Voluminis evolutionis commendata 80μL est.
C: Accipe1.4mL urinae specimen probetur et centrifugium in 12000rpm pro 5 minutis;supernatantem abiicias (commendatur ut 10-20μL supernatantis ab imo tubi centrifugii custodiant), et 200μL salinarum normalium resuspendendi, sicut specimen probandum, adde.Extractio subsequens administrari potest cumQIAamp DNA Mini Kit(51304) by QIAGEN or Macro & Micro-test Viral DNA/RNA Column (HWTS-3020-50).Extractio procedendum est secundum instructiones ad usum.Extractio specimen volumen est 200μL, ac commendatur elutionis volumen 80μL.
2. Swab Cervical / vaginal swab sample
A: Accipe 1mL specimen ut probetur in 1.5mLof tubus centrifugium;etcentrifuge ad 12000rpm pro V minuta. Discard supernatantem (commendatur ut 10-20μL supernatantis ab imo tubi centrifugii custodiant), 100μL emissionis reagentis specimen adde, et deinde iuxta instructiones ad usum Macronis & Micro-test Reagentis (Sample Release HWTS-3005-8).
B: Extractio agi potest cum Macrone & Micro-teste Virali DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (quod cum Macro adhiberi potest. & Micro-test Automaticum Acidum Nuclei Extractor (HWTS-3006C, HWTS-3006B)) Jiangsu Macro & Micro-test Med-Tech Co., Ltd. stricte secundum instructiones ad usum.Extractum specimen voluminis 200μL est, et volumen commendabile 80μL est.
C: Extractio cum QIAamp DNA Mini Kit (51304) deduci potest per QIAGEN vel Macro & Micro-test Viral DNA/RNA Columna (HWTS-3020-50).Extractio procedendum est secundum instructiones ad usum.Extractio voluminis specimen 200 µL est, et volumen commendabile est .80 μL.
3、Cervical Swab/Vaginal Swab
Ante sampling, utere bombicis swab ut excessus secretiones e cervice leniter detergant, et alio swab infiltrato bombacio cum solutione cellae conservationis vel solutione cellae exfoliatae ceruicis sampling, ut mucosa ceruicis adhaereat et turn horologico 3-5 circumeunt, obtineat. cellulis ceruicis exfoliatis.Bombacio swab vel peniculus lente eximito;etmitte in specimen tubo cum 1mL sterilis normalis SAL. Apostquam plene lotis, exprime swab vel penicillo bombicis contra murum tubo siccare et abjicere, obstringere pileum tubum, et notare nomen specimen (vel numerum) et typus in tubi specimen.
