XXVIII Genera Virus Papilloma Humani Periculi Alti (16/18 Typificationes) Acidi Nucleici
Nomen producti
HWTS-CC006A-28 Genera Virus Papillomae Humani Periculi Alti (Typificatione 16/18) Instrumentum Detectionis Acidi Nucleici (PCR Fluorescens)
Epidemiologia
Cancer cervicis uteri est unus e tumoribus malignis tractus genitalis feminini frequentissimis. Studia demonstraverunt infectiones persistentes HPV et infectiones multiplices unam ex causis principalibus cancri cervicis uteri esse. Nunc, curationes efficaces agnitae adhuc desunt pro cancro cervicis uteri ab HPV causato, ergo detectio et praeventio infectionis cervicis ab HPV causatae clavis est ad cancrum cervicis uteri prohibendum. Magni momenti est examen diagnosticum aetiologicum simplex, specificum et celerem ad diagnosim clinicam et curationem cancri cervicis uteri instituere.
Canalis
| Mixtura Reactionis | Canalis | Typus |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | XXXI, XXXIII, XXXV, XXXIX, XLV, LI, LII, LVI, LVIII, LIX, LXVI, LXVIII | |
| CY5 | Imperium Internum | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | XXVI, XLIV, LXI, LXXXI | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Imperium Internum |
Parametri Technici
| Repositorium | Liquidum: ≤-18℃ |
| Tempus conservationis | Duodecim menses |
| Typus Speciminis | Peniculus cervicalis, peniculus vaginalis, urina |
| Ct | ≤28 |
| CV | <5.0% |
| Limitatio Detectionis (vel Limitatio Detectionis) | 300 Exemplaria/mL |
| Instrumenta Applicabilia | Systema PCR Temporis Realis 7500 Biosystems Applied Systema PCR Temporis Realis Celeris Applied Biosystems 7500 QuantStudio®Quinque Systemata PCR Temporis Realis Systema PCR Temporis Realis SLAN-96P Cyclus Lucis®Systema PCR in Tempore Reali 480 Systema Detectionis PCR in Tempore Reali LineGene 9600 Plus MA-6000 Cyclator Thermalis Quantitativus Temporis Realis Systema PCR Temporis Realis BioRad CFX96 Systema PCR Temporis Realis BioRad CFX Opus 96 |
Fluxus Operis
Reagentium extractionis commendatum: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (quod cum Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) adhiberi potest) a Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Adde 200μL solutionis salinae normalis ad pellet resuspendendum in gradu 2.1, et deinde extractio peragenda est secundum instructiones usus huius reagentis extractionis. Volumen elutionis commendatum est 80μL.
Reagentium extractionis commendatum: QIAamp DNA Mini Kit (51304) vel Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Adde 200μL solutionis salinae normalis ad resuspendendum sedimentum in gradu 2.1, et deinde extractio peragenda est secundum instructiones ad usum huius reagentis extractionis. Volumen extractorum omnium exemplorum est 200μL, et volumen elutionis commendatum est 100μL.
