Human CYP2C19 Gene Polymorphismus
Product nomen
HWTS-GE012A-Humanum CYP2C19 Gene Polymorphismus Deprehensio Kit (Fluorescens PCR)
Testimonium
CE/TFDA
Epidemiologia
CYP2C19 una e maximis medicamentis metabolizationis enzymes in familia CYP450 est.Multae subiectae endogenae et circa 2% medicamentorum clinicorum a CYP2C19 metabolized sunt, sicut metabolismi antiplateletae aggregationis inhibitores (velut clopidogrel), sentinae inhibitores (omeprazole), anticonvulsantes, etc. CYP2C19 polymorphismi gene etiam differentias habent in facultate metabolizationis. affinis medicinae.Haec puncta mutationum *2 (rs4244285) et *3 (rs4986893) causant detrimentum enzyme activitatis in CYP2C19 gene et debilitate metabolicae subiecti facultatis, et sanguinem retrahitur auget, ita ut reactiones pharmaca adversas causant ad sanguinis defectus.*17 (rs12248560) enzyme activum enzyme a gene CYP2C19 encyclo augere potuit, effectio metabolitae activae, et aggregationis inhibitionis laminae augere et periculum sanguinis augere.Pro homine lento metabolismi medicamentorum, normales doses diu graves toxici et effectus laterales facient: maxime damnum iecoris, damnum systematis hematopoieticum, systematis nervosi centralis damnum, etc., quae in gravibus casibus ad mortem ducere possunt.Secundum singulas differentias metabolismi medicamento respondente, plerumque in quattuor phaenotypos dividitur, nempe metabolismum ultra-celerium (UM, 17/*17,*1/*17), metabolismi ieiunium (RM, *1/*1 ) metabolismus intermedius (IM, *1/*2, *1/*3), metabolismus tardus (PM, *2/*2, *2/*3, *3/*3).
Channel
| FAM | CYP2C19*2 |
| CY5 | CYP2C9*3 |
| ROX | CYP2C19*17 |
| VIC/HEX | IC |
Technical Parameters
| Repono | Liquid: ≤-18℃ |
| Fasciae vita | XII mensibus |
| Specimen Type | Nova EDTA anticoagulata sanguine |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Specification | Nulla crux-reactivity cum aliis sequentiis maxime consistentibus (CYP2C9 gene) in genome humano.Mutationes CYP2C19*23, CYP2C19*24 et CYP2C19*25 situs extra detectionem amplitudinis huius ornamenti nullum effectum habent in deprehensione huius ornamenti effectum. |
| Applicabilis Instrumenta | Acta Biosystems (VII)D Real-time PCR Systems Acta Biosystems (VII)D Fast Verus-Tempus PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-time PCR Systems LightCycler®480 Real-time PCR systema LineGene 9600 Plus Real-time PCR Detection System MA-6000 Real-time Quantitatis Thermal Cycler BioRad CFX96 Real-time PCR System BioRad CFX Opus 96 Real-time PCR System |
Opus O
Commendatur extraction reagens: Macro & Micro-test Generalis DNA/RNA Kit (HWTS-3019) (quod adhiberi potest cum Macro & Micro-test Automatico Nucleico Acido Extractor (HWTS-EQ011)) ab Jiangsu Macro & Micro-test Med-Tech Co., Ltd. extractio secundum mandatum extrahi debet.Extractio voluminis specimen 200μL est, et volumen commendabile est 100μL.
Commendatur extraction reagens: Veneficus DNA Purificationis Genomic Ornamentum (Catalogue No.: A1120) per Promega, Acidum nuclei extractionis seu Purificationis Reagenti (YDP348) Tiangen Biotech (Beijing) Co.,Ltd.extrahi debet secundum instructiones extractionis et volumen commendatur extractio est 200 µL et commendatur elutionis volumen 160 µL.
