Staphylococcus Aureus and Methicillin-Resistentes Staphylococcus Aureus (MRSA/SA)
Product nomen
HWTS-OT062 Staphylococcus Aureus et Methicillin-Resistentes Staphylococcus Aureus (MRSA/SA) Nucleic Acidum Detection Ornamentum (Fluorescens PCR)
Testimonium
CE
Epidemiologia
Staphylococcus aureus unus est e bacteria pathogenica magnae infectionis nosocomialis.Staphylococcus aureus (SA) ad staphylococcum pertinet et bacteria gram-positiva repraesentativa est, quae varias toxins enzymes et incursiones producere potest.Bacteria notas habent dilatae distributionis, pathogenicitatis validae et magnae resistentiae.nucleasus thermostus gene (nuc) valde conservatus est gene staphylococci aurei.
Channel
| FAM | methicillin repugnans gene |
| ROX | Internum Imperium |
| CY5 | staphylococcus aureus nuc gene |
Technical Parameters
| Repono | ≤-18℃ & tuta ab lumine |
| Fasciae vita | XII mensibus |
| Specimen Type | sputum, cutis et textus mollis infectio exemplaria et exempla nasi swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bacteria methicillino-repugnans.Cum ornamentum nationalis LoD detegit, 1000/mL staphylococcus aureus deprehendi potest |
| Specification | Crux-reactivitatis experimentum ostendit hoc ornamentum non habere reactivum crucis cum aliis pathogenis respiratoriis sicut methicillino-sensitivo staphylococcus aureus, staphylococcus coagulaso-negativus, staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae; mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Applicabilis Instrumenta | Acta Biosystems (VII)D Real-time PCR Systems QuantStudio®5 Real-time PCR Systems SLAN-96P Real-time PCR Systems LightCycler®480 Real-time PCR system LineGene 9600 Plus Real-time PCR Detection System MA-6000 Real-time Quantitatis Thermal Cycler BioRad CFX96 Real-time PCR System BioRad CFX Opus 96 Real-time PCR System |
Opus O
Optio 1 .
Macro & Micro-test Genomic DNA/RNA Kit (HWTS-3019) by Jiangsu Macro & Micro-test Med-Tech Co., Ltd. adhiberi potest cum Macro & Micro-test Automatico Nucleico Acido Extractor (HWTS-3006C, HWTS- 3006B).200 µL de salinis normalibus ad praeproperas processus addere, et vestigia subsequentes secundum instructiones extrahi debent, et volumen commendatur elution 80µL.
Optio II.
Macro & Micro-test Sample Reagent Reagent (HWTS-3005-8) Jiangsu Macro & Micro-test Med-Tech Co, Ltd. Adde 1mL salinarum normalium in praecipiti loto cum salino normali, tum bene misce.Centrifugium ad 13,000r/min per 5 minuta, supernatantem (reserve 10-20µL supernatantem), sequere instructiones ad extractionem subsequentem.
Commendatur extraction reagens: Acidum nuclei extractio seu Purificatio Reagenti (YDP302) by Tiangen Biotech (Beijing) Co.,Ltd.Extractio stricte peragenda est secundum gradum instructionis 2 manualis.Commendatur RNase et DNase-aqua libera uti ad elutionem voluminis 100µL.
