Quindecim Genera mRNA Genorum Papillomavirus Humani Periculi Alti
Nomen producti
HWTS-CC005A-15 Genera Papillomavirus Humani Periculi Alti E6/E7 Geni mRNA Detectionis Instrumenti (PCR Fluorescens)
Epidemiologia
Cancer cervicis inter frequentissima genera cancri feminei toto orbe terrarum numeratur, cuius incidentia arcte cum papillomaviris humanis (HPV) coniungitur, sed parva tantum pars infectionum HPV in cancrum evolvi potest. HPV magni periculi cellulas epitheliales cervicales inficit et duas oncoproteinas, E6 et E7, producit. Haec proteina varietatem proteinorum cellularum (sicut proteinas tumoris suppressorias pRB et p53) afficere, cyclum cellularem prolongare, synthesin DNA et stabilitatem genomi afficere, et responsa immunia antiviralia et antitumoralia impedire potest.
Canalis
| Canalis | Pars | Genotypus probatus |
| FAM | HPV Reaction Buffer 1 | HPV 16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Genum β-actini humani | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Genum INS humanum |
Parametri Technici
| Repositorium | Liquidum: ≤-18℃ |
| Tempus conservationis | Novem menses |
| Typus Speciminis | Peniculus cervical |
| Ct | ≤38 |
| CV | <5.0% |
| Limitatio Detectionis (vel Limitatio Detectionis) | Exemplaria 500/mL |
| Instrumenta Applicabilia | Systema PCR Temporis Realis Applied Biosystems 7500Systema PCR Temporis Realis Celeris Applied Biosystems 7500 Systema PCR Temporis Realis QuantStudio®5 Systema PCR Temporis Realis SLAN-96P Systema PCR in Tempore Reali LightCycler®480 Systema Detectionis PCR in Tempore Reali LineGene 9600 Plus MA-6000 Cyclator Thermalis Quantitativus Temporis Realis Systema PCR Temporis Realis BioRad CFX96 Systema PCR Temporis Realis BioRad CFX Opus 96 |
Fluxus Operis
Reagentium extractionis commendatum: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) a Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Extractio stricte secundum instructiones usus peragenda est. Volumen elutionis commendatum est 50μL. Si specimen non plene digestum est, ad gradum 4 ad redigestionem reduce. Deinde secundum instructiones usus proba.
Reagentium extractionis commendatum: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Extractio secundum instructiones usus stricte peragenda est (in gradu 5, concentrationem solutionis operantis DNaseI duplica, id est, 20 μL solutionis stock DNaseI RNase-Free (1500U) in novum tubum centrifugae RNase-Free sume, 60 μL solutionis RDD buffer adde, et leniter misce). Volumen elutionis commendatum est 60 μL. Si exemplum non plene digestum est, ad gradum 5 ad redigestionem redde. Deinde secundum instructiones usus proba.
