15 Type of Summus periculum Humani Papillomavirus E6/E7 Gene mRNA

Description:

Hoc ornamentum ad detectionem qualitativum 15 summi periculi humani papillomaviri (HPV) E6/E7 gene variante expressionis gradus in cellulis exfoliatis cervicis feminae destinatur.


Product Detail

Product Tags

Product nomen

HWTS-CC005A-15 Types of High-Periculum Humanum Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)

Epidemiologia

Cancer cervicus est una e communissimis generibus cancere muliebris in orbe terrarum, et eius eventus prope papillomaviros humanos (HPV), sed parva proportio infectionum HPV in cancrum evolvere potest.Summus periculum HPV inficit cellulas epitheliales cervicales et duos oncoproteinos producit, E6 et E7.Hic interdum potest varias servo cellulosos afficere (qualis est tumor suppressoris servo pRB et p53), cyclum cellularum prolongare, synthesim DNA et stabilitatem genome afficere, et intercedere responsionibus immunibus antiviralibus et antitumoribus.

Channel

Channel Component Genotype temptavit
FAM HPV Reactio Buffer 1 HPV16、31、33、35、51、52、58
VIC/HEX Humanum β-actin gene
FAM HPV Reactio Buffer 2 HPV 18、39、45、53、56、59、66、68
VIC/HEX Human INS gene

Technical Parameters

Repono Liquid: ≤-18℃
Fasciae vita IX mensibus
Specimen Type Cervical swab
Ct ≤38
CV 5.0%
LoD 500 Copia/mL
Applicabilis Instrumenta Acta Biosystems (VII)D Real-time PCR SystemActa Biosystems (VII)D Fast Verus-Tempus PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-time PCR Systems

LightCycler®480 Real-time PCR systema

LineGene 9600 Plus Real-time PCR Detection System

MA-6000 Real-time Quantitatis Thermal Cycler

BioRad CFX96 Real-time PCR System

BioRad CFX Opus 96 Real-time PCR System

Opus O

Extraction reagens commendatur: Macro & Micro-test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) ab Jiangsu Macro & Micro-test Med-Tech Co. .Voluminis elutionis commendatum 50μL est.Si specimen non perfecte concoctum est, redde illud ad redigendum 4 gradum.Et tunc probate secundum mandatum utendi.

Commendatur extraction reagens: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Extractio facienda est secundum instructiones ad usum stricte (In gradu V, duplex intentio solutionis DNaseI laborantis, hoc est, accipe 20μL ex RNase-Free DNaseI (1500U) solutionem stirpis in novam RNase-Free centrifugium tubum; adde 60μL ex RDD quiddam et leniter misce).Voluminis evolutionis commendata 60μL est.Si specimen non perfecte concoctum est, redde illud ad 5 gradus redigendis.Et tunc probate secundum mandatum utendi.


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